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Fig. 1 | Cancer Cell International

Fig. 1

From: Myc-associated zinc-finger protein promotes clear cell renal cell carcinoma progression through transcriptional activation of the MAP2K2-dependent ERK pathway

Fig. 1

MAZ expression is increased in ccRCC and correlates with poor prognosis. A Hematoxylin and eosin staining of tumor (T) and normal (N) kidney tissues. Scale bar  =  50 μm. B Immunohistochemical staining was used to detect the expression of MAZ in tumor (T) and normal (N) kidney tissues. Scale bar  =  25 μm. C The levels of MAZ protein in tumor (T) and normal (N) kidney tissues were detected by Western blot. D Quantitative analysis of B. E The expression of MAZ mRNA was detected by qRT-PCR in tumor (T, n  =  45) and normal kidney (N, n  =  45) tissues. (F) MAZ mRNA levels in tumor and normal kidney tissues from the TCGA database (http://ualcan.path.uab.edu/analysis.html). G Kaplan–Meier analysis was used to analyze the overall survival of ccRCC patients in the TCGA database with low (n  =  130) and high (n  =  130) MAZ levels (cutoff value is 25%, http://www.oncolnc.org/). H Western blot analysis was used to detect the level of MAZ protein in 293A and RCC cell lines (A498, 786–0, SW839, and Caki-1). I Quantitative analysis of G. J qRT-PCR was performed to detect the MAZ mRNA level in the above cell lines. All data are expressed as the mean  ±  SEM from three independent experiments. *P  <  0.05, **P  <  0.01, ***P  <  0.001 vs. the corresponding controls

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