Fig. 3

The MAP2K2/ERK pathway mediates MAZ-regulated cell proliferation. A Caki cells were transfected with pKLO and shMAZ, and then a Western blot analysis was used to detect the indicated protein expression. B Quantitative analysis of A. C Caki cells were transfected with the pKLO, shMAZ, pWPI, and oeMAZ vectors and then qRT-PCR was used to detect the mRNA levels of upstream ERK-related genes. D SW839 and Caki cells were transfected with the pWPI and oeMAZ vectors and were then treated with the ERK pathway inhibitor FR180204 for 24 h. Western blot was used to detect MAP2K2 and p-ERK expression. E Quantitative analysis of D. F, G Cells were transfected with the pWPI or oeMAZ (F) and pLKO or shMAZ (G) and treated with FR180204; cell viability was measured by MTT assay. All data are expressed as the mean  ±  SEM from three independent experiments. *P  <  0.05, **P  <  0.01, ***P  <  0.001 vs. the corresponding controls