Fig. 5

MAZ promotes the transcription of MAP2K2 in ccRCC cells. A qRT-PCR was used to detect MAP2K2 expression in the above indicated cell lines. B Potential binding site of MAZ in the MAP2K2 promotor. C ChIP-qPCR was used to measure the binding of the transcription factor MAZ to the MAP2K2 promoter region in Caki-1 cells. D Cells were co-transfected with the MAP2K2 promoter-luciferase reporter and pWPI or oeMAZ, transfected with the oeMAZ vector and treated simultaneously with FR180204, and then luciferase reporter assays were performed. E SW839 and Caki cells were transfected with the pKLO, shMAZ, pWPI, and oeMAZ vectors, and then Western blot was performed to detect the MAP2K2 protein level. F Quantitative analysis of E. All data are expressed as the mean  ±  SEM from three independent experiments. *P  <  0.05, **P  <  0.01, ***P  <  0.001 vs. the corresponding controls