Fig. 6

The MAZ/MAP2K2/ERK axis promotes ccRCC progression. A SW839 and Caki cells were transfected with the pKLO, shMAP2K2, pWPI, and oeMAP2K2 vectors and then subjected to qRT-PCR to determine the MAP2K2 mRNA level. B SW839 and Caki cells were transfected as in A, and the MAP2K2 protein level was detected by Western blot. C SW839 and Caki cells were transfected with the indicated vectors and then Western blot was performed to determine the protein levels of MAP2K2, p-ERK, and cyclin D1. D Quantitative analysis of C. E Cells were prepared as in C, and cell viability was measured by MTT assay. All data are expressed as the mean  ±  SEM from three independent experiments. *P  <  0.05, **P  <  0.01, ***P  <  0.001 vs. the corresponding controls