Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: RAMS11 promotes CRC through mTOR-dependent inhibition of autophagy, suppression of apoptosis, and promotion of epithelial-mesenchymal transition

Fig. 6

The knockdown of RAMS11 inhibited EMT markers and transcription factors in CRC cells. The EMT markers and transcription factors expressions were evaluated by western blotting in A–F HCT-116 and G–L SW480 cells. The Dsi-RAMS11 mediated knockdown significantly enhanced the expression of E-cadherin in both B HCT-116 and H SW480 cells compared to Dsi-NC. On the other hand, Dsi-RAMS11 significantly decreased mesenchymal markers N-cadherin and vimentin expressions in C, D HCT-116 and I, J SW480 cells. In addition, EMT regulated transcription factors Sox2 and Snail expressions were significantly downregulated in E, F HCT-116 and K–L SW480 cells after Dsi-RAMS11. The data was shown as relative expression mean ± SEM compared to Dsi-NC group using β-actin as internal control. (*P < 0.05, **P < 0.01, ***P < 0.001 and n = 4)

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us