Fig. 2From: Metformin exerts anti-AR-negative prostate cancer activity via AMPK/autophagy signaling pathwayMetformin blocked cell cycle at G0/G1 phase. a, b Cell cycle distribution of metformin-treated cells was detected by flow cytometry. c The protein expression associated with the cell cycle was determined with Western blot analysis. The relative cyclin D1 and PCNA levels were normalized to that of α-Tubulin. p-ERK/ERK is shown. Numbers under the bands are the relative expression values to that of α-Tubulin, except for p-ERK. Data are presented as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group. Ctr, Control; Met, MetforminBack to article page