Fig. 7

Detection of LTB4R (A) and GNRH1 (B) mRNA expression levels in ccRCC cells and normal kidney cells by qRT-PCR. GNRH1 and LTB4R expression in cancer cells is clearly higher than normal kidney cells. Overexpressed GNRH1 and LTB4R increased GNRH1 and LTB4R protein by western blot analysis (C, D). GAPDH abundance was used as an internal control. Immunofluorescence staining of LTB4R (E) and GNRH1 (F). **P < 0.01