Fig. 8

Detection of LTB4R (A) and GNRH1 (B) mRNA expression levels in 15 pairs of ccRCC and para-cancer tissues by qRT-PCR. GNRH1 and LTB4R expression in ccRCC tissues is clearly higher than para-cancer tissues. Overexpressed GNRH1 and LTB4R increased GNRH1 and LTB4R protein by western blot analysis (C, D). GAPDH abundance was used as an internal control. E, F Analyze the differential expression of LTB4R and GNRH1 in ccRCC and adjacent tissues by IHC experiments. **P < 0.01