Fig. 2

NCK1-AS1 binds to miR-526b-5p. A, B Subcellular fractionation and FISH assays were conducted to determine the localization of NCK1-AS1 in melanoma cells. C RIP assays were performed to examine whether NCK1-AS1 exists in Ago2-RISCs. D RNA pull down assays were performed to screen out the downstream gene of NCK1-AS1. E RIP assays demonstrated that NCK1-AS1 and miR-526b-5p coexisted in RISCs. **p < 0.01