Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: NCK1-AS1 promotes the progression of melanoma by accelerating cell proliferation and migration via targeting miR-526b-5p/ADAM15 axis

Fig. 3

NCK1-AS1 binds to miR-526b-5p to regulate ADAM15. A Expressions of 7 mRNAs (MPC2, MYO5C, CLN6, ADAM15, ABHD14B, PPA2 and CORO2A) in the transfected melanoma cells with miR-526b-5p mimics were analyzed by RT-qPCR. B, C RIP and RNA pull down assays validated the interaction among NCK1-AS1, miR-526b-5p and ADAM15. D, E The binding sites of miR-526b-5p and NCK1-AS1/ADAM15 were predicted by the starBase. The binding between miR-526b-5p and NCK1-AS1/ADAM15 in melanoma were verified by luciferase reporter assays. F Luciferase reporter assays were conducted in A-375, M21, HEK-293 T cells which were transfected with NC mimics, miR-526b-5p mimics or co-transfected with miR-526b-5p mimics and overexpressing NCK1-AS1. **p  <  0.01

Back to article page