Fig. 3

SHP2 inhibition suppressed the proliferation of LUAD cells. A The IC50 of SHP099 in PC9 cells was 20.100 and 7.536 at 4 and 24 h, respectively. B The IC50 of SHP099 in PC9GR cells was 24.670 and 8.900 at 4 and 24 h, respectively. C, D SHP2 over-expressing PC9GR and PC9 cells showed aggressive proliferation than SHP2 knock-down cells after 24 and 48 h. E, colony formation assay showed that larger residual clones were established by PC9 and PC9GR cells with high expression of SHP2 compared with parental cell lines, while smaller residual clones were established in PC9 and PC9GR cells with low expression of SHP2. Each experiments was repeated 3 times