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Fig. 4 | Cancer Cell International

Fig. 4

From: SHP2 inhibition enhances the anticancer effect of Osimertinib in EGFR T790M mutant lung adenocarcinoma by blocking CXCL8 loop mediated stemness

Fig. 4

The stemness of LUAD cells were damaged by SHP2 inhibition. A The percentage of CD133+ CSCs increased from 7.013 ± 0.560 % of parental PC9GR cells to 11.767 ± 0.801 % of SHP2 over-expressed cells, while, the proportion of CD133+ CSCs decreased to 2.973 ± 0.346 % in SHP2 knock down cells. Meanwhile, CD133+ LUAD CSCs in SHP2 over-expressed PC9 cells increased from 3.697 ± 0.092 % of parental cells to 8.370 ± 0.607 %, while the proportion of CD133+ CSCs decreased to 1.467 ± 0.319 % when knocking down SHP2. B PC9 and PC9GR cells with high SHP2 formed bigger and more spheres than homologous parental cells in soft Agar culturing, while low SHP2 expressed cells showed impaired ability to form spheres. C PC9 and PC9GR cells with high SHP2 formed bigger and more spheres than their parental cells, while low SHP2 expressed PC9 and PC9GR cells had a significantly attenuated ability to form spheres. D After 7 days of culture, the proportion of CSCs in SHP2 high-expressing PC9GR cells was significantly enriched from 10.410 ± 0.506 % of the parental cells to 64.833 ± 2.188 %, and the CSCs of low SHP2 expressed PC9GR cells was also significantly enriched after 7 days which increased from 2.910 ± 0.260 % of the parental cells to 46.267 ± 1.933 %. E 50,000, 5000 and 500 high and low SHP2 modified PC9 and PC9GR cells were inoculated into Balb/c-Nu nude mice, respectively, and the SHP2 cells showed aggressive capability of generating tumors. F Phosphorylated protein of GSK3β and βCatenin were reduced in low SHP2 PC9GR cells. Each experiment was repeated 3 times

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