Fig. 2

BRD4 inhibition downregulates JOSD1 in HNSCC. a TCGA-HNSCC database analysis showed that both JOSD1 and BRD4 were overexpressed in HNSCC tissues. b BRD4 correlated positively with JOSD1, which was analyzed by GEPIA. c, d The effect of JQ1 on JOSD1 was detected by using qRT-PCR and western blot assay. The dosage of JQ1, 250 nM. e, f The mRNA and protein expression of BRD4 and JOSD1 were measured in the HNSCC cells expressing shBRD4. g, h The effect of BRD4 overexpression of JOSD1 was measured by using qPCR and western blot assays. i The representative images of IHC staining of BRD4 and the analysis of the correlation between BRD4 and JOSD1 in HNSCC tissues. Scale bar, 50 μm. Data in this figure, mean ± SD, *P < 0.05, **P < 0.01. TPM, transcripts per million