Fig. 3

LncRNA HAND2-AS1 functions as a sponge for miR-146. A miR-146 expression in bladder cancer specimens was determined by qRT-PCR. *P < 0.01, vs. adjacent tissues, n = 32. B miR-146 expression in the indicated cell lines was determined by qRT-PCR. *P < 0.05, n = 3. C The correlation of miR-146 and HAND2-AS1 expression. n = 32. D Schematic diagram of the putative binding sites of HAND2-AS1 and miR-146. E The relative activity of the miR-146 promoter in the indicated cells was determined by dual-luciferase assay. miR-146wt, miR-146 wild-type promoter. miR-146mut, miR-146 mutated promoter. *P < 0.05, vs. control, n = 3. #P < 0.05, vs. si-NC, n = 3. F The expression of miR-146 wt or miR-146mut in 5637 cells expressing HAND2-AS1 or si-HAND2-AS1 was accessed by qRT-PCR. *P < 0.05, vs. control, n = 3. #P < 0.05, vs. si-NC, n = 3