Fig. 5

The overexpression of lncRNA HAND2-AS1 attenuates cell proliferation and enhances apoptosis by releasing RARB from miR-146. A The proliferation of the indicated cells was accessed by MTT assay. *P < 0.05, vs. control, n = 3. B The apoptosis of the indicated cells was detected by FITC-PI stain, followed by flow cytometry analysis. Representative images of three independent experiments were shown. *P < 0.05 vs. vector. ns, no significance. C The expression of HAND2-AS1, miR-146, RARB, and Caspase 3 in the indicated cells were measured by qRT-PCR. *P < 0.05 vs. control, n = 3. ns, no significance. D The expression of HAND2-AS1, miR-146, RARB, and Caspase 3 in the indicated cells were measured by western blot