Fig. 7

Wound healing assay and transwell invasion assay. A RT-PCR analysis of gene expression of LOC641518 and LEF1 in MCF-7 and MDA-MB-231 cells; B RT-PCR analysis of gene expression of LOC641518 and LEF1 in MCF-7 and MDA-MB-231 cells after knocking down LOC641518. B Wound closure was shown at 48 h post scratch (200×) after LOC641518 knocking down in MCF-7 and MDA-MB-231 cells, and over-expression LEF reverses its effect on wound closure. Cell migration was assessed by recover of the scratch. The area of the wound was measured at the two time points in every group, and % reduction of initial scratch area was compared with scramble; and the results were expressed as percentage relative to the corresponding scramble control. C Transwell invasion assay. MCF-7 and MDA-MB-231 cells penetrating the membrane were fixed and 0.1% crystal violet stained after 24 h as described in experimental procedures, and cell number penetrating the membrane were counted for each group. *P < 0.05, **P < 0.01. Data were the means of three measurements and the bars represented SD of the mean