Fig. 2

CSN5 knockdown inhibits cell growth in vitro and in vivo. Depicted are Western blot analysis of indicated proteins (A), CCK8 tests (B, C), clone formation (D) and cell cycle analysis (E, F) in Siha and Hela cells with CAS9 CSN5 knockdown (gCSN5), and a non-specific guide RNA as control (gNS). (G, H, I) Hela cells with gCSN5 and controls were subcutaneously injected into nude mice (n = 7 mice for each group). Tumor volumes were measured at different time points and tumors were harvested and weighed after 24 days. (Mean with S.E.M. *, p < 0.05; **, p < 0.01; ***, p < 0.001)