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Fig. 4 | Cancer Cell International

Fig. 4

From: β-Catenin/LEF-1 transcription complex is responsible for the transcriptional activation of LINC01278

Fig. 4

LINC01278 interacts with β-catenin. A The protein expression of β-catenin in TPC-1 and BCPAP cells which were transfected with LINC01278 siRNA (LINC-KD) or overexpression plasmid (LINC01278). B The luciferase activity of TPC-1 and BCPAP cells which were co-transfected with TOP-Flash and LINC01278 siRNA (LINC-KD) or overexpression plasmid (LINC01278). C The enrichment of LINC01278 in β-catenin protein by using RNA immunoprecipitation and qRT-PCR. ACTB was used as the control. D LINC01278 precipitated β-catenin in TPC-1 and BCPAP cells detected by RNA pulldown and western blot. *P < 0.05

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