Fig. 3

The relationship of circFBXL5 and miR-216b. A The binding sequence of circFBXL5 and miR-216b. B, C Luciferase activity was detected in cells with co-transfection of circFBXL5-WT or circFBXL5-MUT and miR-216b mimic or miR-con. D circFBXL5 expression in cells with transfection of biotin-miR-con or biotin-miR-216b after RNA pull-down. E miR-216b abundance in breast cancer tissues and normal tissues (n = 39). F circFBXL5 and miR-216b expression association in breast cancer tissues. G miR-216 abundance in breast cancer cell lines (MDA-MB-453 and MDA-MB-231) and normal human breast epithelial cell line MCF-10A. H miR-216 abundance in 5-FU-resistant and sensitive MDA-MB-231 and MDA-MB-453 cells. I, J miR-216 expression in cells with transfection of vector, circFBXL5 overexpression vector, si-circFBXL5 or si-con. Mock: non-transfected group. ^*P < 0.05