Fig. 5
From: CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis
The relationship of miR-216b and HMGA2. A The binding sequence of miR-216b and HMGA2. B, C Luciferase activity was examined in cells with co-transfection of HMGA2-WT or HMGA2-MUT and miR-216b mimic or miR-con. D HMGA2 abundance in cells with transfection of biotin-miR-con or biotin-miR-216b after RNA pull-down. E HMGA2 protein expression in MDA-MB-453, MDA-MB-231 and MCF-10A cells. F HMGA2 abundance in 5-FU-resistant and sensitive MDA-MB-231 and MDA-MB-453 cells. G, H HMGA2 protein level in cells with transfection of miR-con, miR-216b mimic, in-miR-con or in-miR-216b. Mock: non-transfected group. *P < 0.05