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Fig. 3 | Cancer Cell International

Fig. 3

From: miR-198 inhibits the progression of renal cell carcinoma by targeting BIRC5

Fig. 3

miR-198 affects cell growth. A Apoptosis assay. A498 and ACHN cells were transfected with the scrambled mimic, miR-198, and miR-198 + FLAG-BIRC5 for 36 h, following which, they were treated with 100 μg/ml of puromycin for 36 h, and analyzed using flow cytometry. B Statistical analysis of the apoptosis rate in A498 cells in (A). **P < 0.01. C Statistical analysis of the apoptosis rate in ACHN cells in (A). ****P < 0.0001. D CCK8 assay of A498 cells. A498 cells were transfected with the scrambled mimic, wild-type miR-198 and miR-198 + FLAG-BIRC5. Then, 5 μL of CCK-8 solution was added to the cells, and cell viability rate was determined using an enzyme marker at 0, 24, and 48 h. *, P < 0.05; **P < 0.01. E CCK8 assay of ACHN cells. ACHN cells were treated as described in (D). *P < 0.05

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