HOXB7 protein expression and location in human HNSCC samples and cell lines were detected by immunohistochemical and immunofluorescence staining, respectively
A–F The immunohistochemical staining of human HNSCC samples. Representative negative staining of HOXB7 in normal oral epithelial (A, B); Representative low expression of HOXB7 in primary human HNSCC sample (C, D); Representative high expression of HOXB7 in primary human HNSCC sample (E, F). The areas marked by black box in the A, C, E images (upper panel) were shown in larger magnification as B, D, F images (lower panel), respectively. Scale bar: 100 μm.
G, H: The immunofluorescence staining of HOXB7 in Cal27 and Fadu cells. HOXB7 was predominantly identifified in nucleus and rarely in cytoplasm in Cal27 (G) and Fadu (H). Nuclei are counterstained with DAPI. Scale bar: 100 μm