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Fig. 2 | Cancer Cell International

Fig. 2

From: MINDY1 promotes bladder cancer progression by stabilizing YAP

Fig. 2

MINDY1 associates with YAP and increases its stability. A An immunofluorescence assay demonstrated that MINDY1 and YAP at least partially colocalized in T24 and UMUC3 cells. B Co-IP assay revealed an association between endogenous MINDY1 and YAP in T24 cells. T24 cells were harvested with RIPA lysis buffer. Co-IP was performed using antibody as indicated. C, D MINDY1 and YAP domain structure and deletion mutants used in the study. E The catalytic domain of MINDY1 interacted with YAP. HEK293 cells were transfected with 2 µg Myc-YAP together with Flag-MINDY1 full length or mutants. After 24 h, cells were harvested with NP-40 lysis buffer. Co-IP was performed using Myc antibody. The possible interacted MINDY1 domains were detected by Flag antibody. F YAP interacted with MINDY1 through its WW terminal. HEK293 cells were transfected with 2 µg Flag-MINDY1 together with Myc-YAP full length or mutants. After 24 h, cells were harvested with NP-40 lysis buffer. Co-IP was performed using Flag antibody. The possible interacted YAP domains were detected by Myc antibody

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