Fig. 3
From: MINDY1 promotes bladder cancer progression by stabilizing YAP
MINDY1 increases YAP stability. A In the presence of the proteasome inhibitor MG132, depletion of MINDY1 did not further decrease the YAP protein level. Bladder cancer cells were transfected with siMINDY1 or siControl. After 48 h, cells were treated with 10 µM MG132 / vehicle for 6 h, cell lysates were prepared for western blot analysis. B T24 cells were transfected with MINDY1 (wild type or C137A) together with MINDY1 siRNA. The YAP protein levels were measured. C MINDY1 depletion decreased YAP half-life in bladder cancer cells. Bladder cancer cells were transfected with siMINDY1 or siControl. After 48 h, cells were treated with 100 µM cycloheximide/vehicle for indicated times. Cell lysates were prepared for western blot analysis. D MINDY1C137A did not increase YAP half-life in HEK293 cells. HEK293 cells were transfected with YAP plasmid and Myc-tag, Myc-MINDY1 or Myc- MINDY1C137A plasmids. After 24 h, cells were treated with 100 µM cycloheximide/vehicle for indicated times. Cell lysates were prepared for Western blot analysis