Fig. 9From: A novel signature constructed by ferroptosis-associated genes (FAGs) for the prediction of prognosis in bladder urothelial carcinoma (BLCA) and associated with immune infiltrationVerification of the crucial ferroptosis-associated genes (FAGs) in the signature. A The mRNA levels of four FAGs (TFRC, SQLE, G6PD and CRYAB) in J82 and SV-HUC1 were detected by QRT-PCR. B The efficiency of TFRC siRNA was validated utilizing western blots. C, D The CCK-8 and colony formation assays indicated that down-regulation of TFRC inhibited cell proliferation in J82 and UMUC3. E TFRC had no effect on the apoptosis of BLCA cells. **P < 0.01; *P < 0.05Back to article page