Fig. 5

FER1L4 required miR-612 to promote PTC cell growth and invasion. A, B Detection of cellular proliferation in K-1 and TPC-1 cells as indicated in CCK8 assays. C Colony formation assays were performed to evaluate the effect of miR-612 on cellular growth. D, E Overexpression of miR-612 induced cell apoptosis and cell cycle arrest at the S phase. F Ectopic expression of miR-612 inhibited PTC cell migration and invasion in transwell assays. G, H Cellular growth curves of K-1 and TPC-1 cells co-transfected with miR-612 mimics and FER1L4 siRNA as indicated by CCK8 assays. I, J Transwell assay was used to determine the migrative ability and invasive ability of PTC cells co-transfected with FER1L4 siRNA and miR-612 inhibitor. K The expression of miR-612 was detected by qRT-PCR analysis in PTC cells co-transfected with FER1L4 siRNA and miR-612 inhibitor. Error bars, mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001