Fig. 2
From: IGHG1 upregulation promoted gastric cancer malignancy via AKT/GSK-3β/β-Catenin pathway
IGHG1 modulation influenced tumor cell proliferative capabilities of gastric cancer cells. A Western Blot analysis on IGHG1 protein expression level differences in gastric cancer cell lines (MKN45, HGC27, MGC-803, BGC-823, AGS, MKN28) and normal gastric epithelial cell line GES-1; B qRT-PCR detection of IGHG1 mRNA expression in gastric cancer cell lines (MKN45, HGC27, MGC-803, BGC-823, AGS, MKN28) and normal gastric epithelial cell line GES-1; C Design and transfection of IGHG1 specific shRNA and IGHG1 overexpression vector into AGS1 and MKN45 cell line respectively; D qRT-PCR analysis on the modulative effects of IGHG1 specific shRNA and overexpression vector on IGHG1 mRNA expression in AGS and MKN45 cell line; E CCK8 assay on AGS1 and MKN45 cellular proliferative capabilities, each cell group was respectively transfected with IGHG1 specific shRNA or IGHG1 overexpression vectors; F Edu assay on AGS1 and MKN45 cellular proliferative capabilities, each cell group was respectively transfected with IGHG1 specific shRNA or IGHG1 overexpression vectors; G, H. Western Blot and qRT-PCR analysis on cyclinD1 and PCNA protein and mRNA expression level in cell groups respectively transfected by IGHG1 specific shRNA and overexpression vectors