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Fig. 6 | Cancer Cell International

Fig. 6

From: Gly-tRF enhances LCSC-like properties and promotes HCC cells migration by targeting NDFIP2

Fig. 6

Overexpression of NDFIP2 partially reverses the HCC-promoting effect of Gly-tRF. A, B The sphere formation assay was performed to verify that the overexpression of NDFIP2 reversed CSCs sphere formation ability caused by Gly-tRF. Magnification, 100 × . C-D. The Transwell assay was performed to verify that the overexpression of NDFIP2 reversed the promotion of Gly-tRF on HCC cell migration. Magnification, 200 × . E, F Wound healing assay was performed to verify that the overexpression of NDFIP2 reversed the promotion of Gly-tRF on HCC cell migration. Magnification, 100 × . G Western blotting was performed to detect the abundance of core markers (N-cadherin, E-cadherin) in EMT. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001

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