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Fig. 2 | Cancer Cell International

Fig. 2

From: LncRNA HOTAIR promotes proliferation and inhibits apoptosis by sponging miR-214-3p in HPV16 positive cervical cancer cells

Fig. 2

HOTAIR plays a growth promoting role in cervical cancer cells. A C-33A, End1/E6E7 and SiHa cells were transfected with si-HOTAIR and si-NC for 24 h, 48 h and 72 h, the cell viability was measured by CCK8 assay. Results showed that the cell viability of End1/E6E7 and SiHa cells were significantly inhibited compared to control cells, but there was no significant change in C-33A. B Cell proliferation was measured in C-33A, End1/E6E7 and SiHa cells transfected with si-HOTAIR and si-NC by EdU incorporation assay. The cell proliferation of End1/E6E7 and SiHa cells were significantly inhibited after HOTAIR knocked down. The representative fields were photographed and counted at × 200 magnification. The cells were counted in five different fields per assay under the microscope. C The cell apoptosis was analyzed by Flow cytometry analysis. Compared with cells transfected with si-NC, the percentage of apoptotic cells in SiHa and End1/E6E7 were significantly increased after transfected with si-HOTAIR. D The cell viability was obvious increasing when overexpressed functional motif of HOTAIR in C-33A cells by CCK8 assay. E The early apoptosis of C-33A cell was significantly decreased after overexpressed HOTAIR-A by flow cytometry analysis. The mean values and standard error were obtained from three independent experiments. *P < 0.05, **P < 0.01

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