miR-214-3p was down regulated expression in HPV16 positive cells and acted as a suppressor gene in cervical cancer cells. A The expression miR-214-3p in End1/E6E7 and SiHa cells was significantly lower than in C-33A cells. B, C C-33A, End1/E6E7 and SiHa cells were transfected with miR-214-3p mimic and negative control for 24 h, 48 h and 72 h, the cell viability was measured by CCK8 assay and cell proliferation was measured by EdU incorporation assay. Results showed that the cell viability and proliferation of End1/E6E7 and SiHa cells were significantly inhibited, but there was no significant change in C-33A cells. The representative fields were photographed and counted at × 200 magnification. The cells were counted in five different fields per assay under the microscope. D Upregulated expression of miR-214-3p increases early apoptotic population in End1/E6E7 and SiHa cervical cancer cells after transfected with miR-214-3p mimic. The mean values and standard error were obtained from three independent experiments. *P < 0.05, **P < 0.01.