Fig. 4

Validation of HOTAIR as a sponge of miR-214-3p in cervical cancer cells. A Bioinformatics analysis of miR-214-3p response elements on HOTAIR sequence. The seed sequences of miR-214-3p (CAGCAGG) were fully complementary to the 1807–1813 bp on HOTAIR. B The activity of luciferase was significantly reduced after co-transfected with miR-214-3p mimics and HOTAIR reporter plasmid in HEK-293 T cells overexpressing miR-214-3p. C Knockdown of HOTAIR significantly upregulated the expression of miR-214-3p in HPV16 positive cells, but not in HPV negative C-33A cells. D HOTAIR-A (contain MRE) obviously inhibited miR-214-3p expression in C-33A cells. E The expression level of miR-214-3p was significantly upregulated when HPV16 E7 was inhibited in End1/E6E7 and SiHa cells, respectively. F The expression level of miR-214-3p was significantly reduced when HPV16 E7 expression was enhanced in C33A, End1/E6E7 and SiHa cells, respectively. The mean values and standard error were obtained from three independent experiments. *P < 0.05, ***P < 0.001