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Fig. 2 | Cancer Cell International

Fig. 2

From: Protopine triggers apoptosis via the intrinsic pathway and regulation of ROS/PI3K/Akt signalling pathway in liver carcinoma

Fig. 2

Protopine induced apoptosis via the mitochondrial pathway in a caspase-dependent manner in liver carcinoma cells. A HepG2 and HuH-7 cells were treated with various doses of protopine for 24 h, then cellular apoptosis was assayed by the flow cytometry. B HepG2 and HuH-7 cells were treated with various doses of protopine for 24 h, then relative caspases activities were measured. C HepG2 and HuH-7 cells were treated with various doses of protopine for 24 h, then cellular lysates were subjected to western blots with indicated antibodies. D HepG2 and HuH-7 cells were pretreated with different caspases inhibitors for 0.5 h, then cells were treated with protopine (40 μM) for another 24 h and cellular apoptosis was measured. E HepG2 and HuH-7 cells were treated with various doses of protopine for 24 h, the expression of Bcl-2 and Bcl-xl was measured by western blots. F HepG2 and HuH-7 cells were treated with various doses of protopine for 24 h, the release of cytochrome c into cytosol was measured by western blots. Data were presented as mean ± SD; *P < 0.05; **P < 0.01

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