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Fig. 4 | Cancer Cell International

Fig. 4

From: Protopine triggers apoptosis via the intrinsic pathway and regulation of ROS/PI3K/Akt signalling pathway in liver carcinoma

Fig. 4

Protopine treatment led to the generation of ROS in liver carcinoma cells. A HepG2 and Huh-7 cells were treated with various doses of protopine for 6 h, intracellular ROS generation was measured by flow cytometer. B HepG2 and Huh-7 cells were treated with various doses of protopine for 6 h, then the content of MDA was measured. C HepG2 and Huh-7 cells were treated with various doses of protopine for 6 h, then levels of LDH were measured. D HepG2 and Huh-7 cells were treated with various doses of protopine for 6 h, SOD activities were measured. E HepG2 and Huh-7 cells were treated with various doses of protopine for 6 h, levels of GPX were measured. F, HepG2 and Huh-7 cells were pre-treated with NAC (10 mM) for 0.5 h, then cells were treated with or without protopine for 24 h, cellular lysates were subjected to western blotting analysis with indicated antibodies. G HepG2 and Huh-7 cells were pre-treated with NAC for 0.5 h then cells were treated with or without protopine for another 24 h, relative caspase-3 activities were measured. H HepG2 and Huh-7 cells were pre-treated with NAC for 0.5 h then cells were treated with or without protopine for another 24 h, then cellular apoptosis was measured. Data were presented as mean ± SD; *P < 0.05; **P < 0.01

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