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Fig. 2 | Cancer Cell International

Fig. 2

From: Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B

Fig. 2

The role of circ-SCMH1 in DDP resistance of OSCC cells in vitro. A, B MTT assay measured cell viability of SCC-15, SCC-15/DDP, CAL-27, and CAL-27/DDP cells. C The half-maximal inhibitory concentration (IC50) of DDP was determined in the dose response curves. DI SCC-15/DDP and CAL-27/DDP cells were transfected with siRNA against circ-SCMH1 (si-circ-SCMH1) or negative control (si-NC) for 48 h, comparing to control cells (without transfection). D RT-qPCR detected circ-SCMH1 level with normalization to GAPDH. E MTT assay determined IC50 of DDP. F Colony formation assay measured number of colonies. G Flow cytometry (FCM) analyzed apoptosis rate. H, I Transwell assays measured numbers of migrated cells and invaded cells. J, K Western blotting examined relative protein expression of Bax and cleaved-caspase-3 with correction with GAPDH. ***P < 0.001 and ****P < 0.0001 from three separate assays

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