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Fig. 4 | Cancer Cell International

Fig. 4

From: Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B

Fig. 4

The reciprocal effect between circ-SCMH1 and miR-338-3p in DDP resistance of OSCC cells in vitro. SCC-15/DDP and CAL-27/DDP cells were transfected with si-NC alone, si-circ-SCMH1 alone or together with anti-RNA against miR-338-3p (anti-miR-338-3p) or the negative control (anti-miR-NC). A RT-qPCR detected miR-338-3p level after transfection with normalization to U6. B MTT assay identified IC50 of DDP after transfection. C Colony formation assay measured number of colonies after transfection. D FCM analyzed apoptosis rate after transfection. E, F Transwell assays measured numbers of migrated cells and invaded cells after transfection. G, H Western blotting examined relative protein expression of Bax and cleaved-caspase-3 with correction with GAPDH. **P < 0.01, ***P < 0.001 and ****P < 0.0001 from three separate assays

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