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Fig. 5 | Cancer Cell International

Fig. 5

From: Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B

Fig. 5

The downstream target of miR-338-3p in OSCC cells. A The starBase v2.0 algorithm showed the complementary binding sites among wild-type of LIN28B 3′UTR (WT-LIN28B 3′UTR), miR-338-3p and mutant-type of LIN28B (MUT-LIN28B 3′UTR). B, C Dual-luciferase reporter assay examined luciferase activity of SCC-15/DDP and CAL-27/DDP cells co-transfected with WT/MUT-LIN28B 3′UTR and miR-338-3p or miR-NC. D, E RIP assays of IgG and Ago2 examined RNA levels of miR-338-3p and LIN28B in cell extracts of SCC-15/DDP and CAL-27/DDP cells. F, G RNA pull-down assay measured LIN28B enrichment in cell extracts of SCC-15/DDP and CAL-27/DDP cells transfected with bio-miR-338-3p or bio-miR-NC. H RT-qPCR measured LIN28B mRNA protein expression and I, J western blotting measured LIN28B protein expression in OSCC tissues in Resistant and Sensitive groups (N = 31) and cell lines (HOK, SCC-15, SCC-15/DDP, CAL-27, and CAL-27/DDP) with normalization to GAPDH. K Pearson’s correlation (r) analysis evaluated the relationship between miR-338-3p and LIN28B expression in OSCC tissues in Resistant group (N = 31). ***P < 0.001 and ****P < 0.0001 from three separate assays

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Cancer Cell International

ISSN: 1475-2867

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