Fig. 4

MALAT1 can promote the expression of HMGA2 by sponging miR-26b in BC cells. A The results from starBase database indicated binding sites between miR-26b and target HMGA2 mRNA 3’UTR. B The expression of HMGA2 in BC tissues was detected by Western blot analysis and immunohistochemistry, n = 68. C The expression of HMGA2 in BC cells was detected by Western blot analysis. D The binding of miR-26b and HMGA2 mRNA was confirmed by the luciferase reporter gene test. E The expression of miR-26b was detected by qRT-PCR. F The expression of HMGA2 in MCF-7 cells was detected by Western blot analysis. G The expression of miR-26b and HMGA2 in BC cells was detected by qRT-PCR after MALAT1 was silenced. H The expression of MALAT1 and HMGA2 in MCF-7 cells was detected by qRT-PCR when miR-26b was overexpressed. *Significant difference (P < 0.05). The above data were all measurement data and expressed as mean ± standard deviation. Paired t-test was used between BC and adjacent tissue samples, and unpaired t-test was used between other two groups. One-way ANOVA with Tukey’s post-test was used for data comparison among multiple groups. All experiments were repeated three times