Fig. 6

lnc-STYK1-2 regulates bladder cancer cell functions through its association with miR-146b-5P. A Relative expression of miR-146b-5p in 5637 and T24 cells transfected with miR-146b-5p inhibitors. The expression of miR-146b-5p was assessed by qRT-PCR. B The proliferation of 5637 and T24 cells after lnc-STYK1-2 silencing and miR-146b-5p inhibitor treatment. The proliferation of 5637 and T24 cells was assessed by MTS. C, D Effects of miR-146b-5p inhibitor treatment on the migration capacities of 5637 and T24 cells transfected with lnc-STYK1-2 siRNAs. E, F Influence of miR-146b-5p inhibitors on the invasion of 5637 and T24 cells with silenced lnc-STYK1-2 expression. The migration and invasion capacities of bladder cancer cells were evaluated using the Transwell system. G Direct association of lnc-STYK1-2 with miR-146b-5p in 293T cells. The binding between lncRNA and microRNA was tested via dual-luciferase reporter assay, and predicted binding sites were shown on the right. NC: negative control; WT: wild type; MUT: mutant type; *P < 0.05; **P < 0.01; ****P < 0.0001