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Fig. 7 | Cancer Cell International

Fig. 7

From: Lnc-STYK1-2 regulates bladder cancer cell proliferation, migration, and invasion by targeting miR-146b-5p expression and AKT/STAT3/NF-kB signaling

Fig. 7

Regulation of ITGA2 and AKT/NFκB/STAT3 signaling by lnc-STYK1-2 and miR-146b-5p interaction. A Relative ITGA2 mRNA levels in bladder cancer cells transfected with lnc-STYK1-2 siRNAs and miR-146b-5p inhibitors. The mRNA levels were detected by the qRT-PCR assay. B Alterations in ITGA2 protein levels in bladder cancer cells induced by lnc-STYK1-2 siRNAs and miR-146b-5p inhibitors. ITGA2 protein contents were analyzed by Western blotting using GAPDH as the internal standard. C The regulative abundances of p65, STAT3, and AKT protein and their phosphorylation levels in the 5637 and T24 cells treated with lnc-STYK1-2 siRNAs and miR-146b-5p inhibitors. Western blotting was performed to analyze the expression and phosphorylation (normalized to total protein) of p65, STAT3, and AKT in bladder cancer cells. NC: negative control; ITGA2: integrin α2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; STAT3: signal transducer and activator of transcription; AKT: protein kinase B; miR-146b: miR-146b-5p; **P < 0.01; ***P < 0.001

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