Fig. 5

6-Paradol-mediated ubiquitination degradation of EGFR leads to inactivate PI3K/AKT signaling pathway. A The effect of 6-Paradol on EGFR mRNA by PCR analysis. B, C The effect of 6-Paradol on protein stability of EGFR was measured using 293 T cells treated with CHX for 1 h or 2 h. D A proteasome inhibitor, MG-132 (5 μM), was used to evaluate whether 6-Paradol was involved in EGFR degradation by a proteasome-dependent route. E Co-immunoprecipitation and SDS-gel electrophoresis were performed to evaluate the levels of EGFR ubiquitination in 293 T cells. F, G Western blot assay was performed to measure the protein expression levels of PI3K/AKT signaling in MIA PaCa-2 and SW1990 cells treated with 6-Paradol and EGFR overexpressed plasmid. H Immunofluorescence analysis of p-AKT levels in the 6-P and NC groups in MIA PaCa-2 and SW1990 cells. **p < 0.01