Fig. 6

EGFR inhibitor enhanced 6-Paradol mediated-inhibition effect on PI3K/AKT signaling activity. A, B Western blot assay was performed to measure the protein expression levels of PI3K/AKT signaling in MIA PaCa-2 and SW1990 cells treated with 6-Paradol, EGFR overexpressed plasmid and Erlotinib (2 nM). C–F Gain- or Lose-Functional experiments were performed to measure the proliferation and migration abilities of pancreatic cancer cells, which were respectively treated with 6-Paradol, EGFR overexpressed plasmid and (or) Erlotinib (2 nM). G, H The protein expression changes of E-cadherin, N-cadherin and Vimentin in Control, 6-P, 6-P + Erlotinib and 6-P + Erlotinib + EGFR groups, respectively. *p < 0.05, **p < 0.01