Fig. 5

circFAM13B is the molecular sponge of miR-212-3p. A, B qRT-PCR assays were performed to determine the expression level of miR-126, miR-5691, miR-146a, miR-212 and miR-520b in HepG2 and Smmc-7721 transfected with overexpression plasmid (circFAM13B) or control vector. C The co-precipitated miR-212 and circFAM13B were subjected to qRT-PCR by RIP experiments in HepG2 and Smmc-7721. D FISH showed that circFAM13B and miR-212 were co-localized in the cytoplasm of HepG2. E, F Schematic illustration of circFAM13B-wt and circFAM13B-mut luciferase reporter vectors. G The relative luciferase activities were detected in HepG2 transfected with circFAM13B-wt or circFAM13B-mut and miR-212 mimics or miR-NC (***P < 0.001, **P < 0.01, *P < 0.05)