Fig. 7

A TargetScan, miRDB and mirDIP were used to predict the potential target genes of miR-212. B, C qRT-PCR assays were performed to determine the expression level of LEMD3, TIMM9, SPPL3, KLF7, E2F5, LEMD3, RPP14, KCNK2, SOX5 and SKAP2 in HepG2 and Smmc-7721 transfected with miR-NC or miR-212 mimic. D Western blotting were performed to determine the expression level of E2F5 in HepG2 and Smmc-7721 transfected with miR-NC or miR-212 mimic. E Schematic illustration of E2F5-wt and E2F5-mut luciferase reporter vectors. F The relative luciferase activities were detected in HepG2 transfected with E2F5-wt or E2F5-mut and miR-212 mimics or miR-NC. G According to Kaplan–Meier Plotter, HCC patients with high mRNA expression level of E2F5 demonstrated poorer prognosis than HCC patients with relatively low mRNA expression level of E2F5 (***P < 0.001, **P < 0.01, *P < 0.05)