Fig. 4

Downregulation of NAT10 inhibited the proliferation of Ca-l27, FaDu and Detroit-562 cell lines and arrested them in S/G2 phase in vitro. a The proliferation rates of Cal-27, FaDu and Detroit-562 cell lines were decreased by the two siRNAs of NAT10. b The IC50 values of Remodelin (an inhibitor of NAT10) were calculated in three cell lines by CCK-8 assay. c Remodelin decreased the proliferation rates of Cal-27, FaDu and Detroit-562 cell lines. d The cell cycle of the three cell lines was arrested in S and G2 phases by NAT10 siRNA, and data were analyzed by FlowJo software. e Three cell lines were transfected with NAT10 siRNA in vitro, and the results indicated no difference in cell apoptosis. All statistical analyses were performed with SPSS 23.0. T tests were performed to compared differences between two groups, and one-way ANOVA was performed to compare differences between three or more groups. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001