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Fig. 2 | Cancer Cell International

Fig. 2

From: DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling

Fig. 2

DDX52 knockdown impairs PCa cell proliferation in vitro. 22RV1 and PC3 cells were infected with lentivirus containing short hairpin RNA (shRNA) against DDX52 or the non-target control (shCON). a The efficiency of knockdown was verified using western blotting. Vinculin was used as the control to measure the loading quantities of the samples for western blotting. b, c 22RV1 and PC3 cells expressing shRNA targeting DDX52 or a control sequence were cultured in 96-well plates for 5 days. The growth rate in each group was measured using the Cell Counting Kit-8 assay (t-test, error bars represent 95% CIs; ***p < 0.001). d The viability of 22RV1 and PC3 cells expressing shRNA or shCON was determined using a colony formation assay. e Histogram showing the number of colonies in each group (t-test, error bars represent 95% CIs; ***p < 0.001)

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