Fig. 5

Protein expression of LYRM4 and Fe-S cluster biosynthesis were significantly increased in LIHC and affected LIHC patient prognosis. a The protein expression levels of LYRM4 were elevated in LIHC (The Human Protein Atlas database). b Representative immunohistochemistry images of LYRM4 in tissue arrays containing human LIHC specimens and matched adjacent normal tissues. Regions in squares are magnified 4× in bottom panels. c Summary statistics of H-score based on only intact and paired specimens (n = 92). d Western blot analysis of LYRM4 in normal hepatocytes and three LIHC cell lines. e–g Box plots showing NDUFS8 (e), POLD1 (f), and PRIM2 (g) mRNA expression levels in LIHC (GEPIA2). h–i Kaplan-Meier diagram of the relationship between POLD1 (h) and PRIM2 (i) gene expression and survival in LIHC patients (GEPIA2). j Protein expression of mitochondrial aconitase ACO2 in LIHC increased (The Human Protein Atlas database). k–l Activities of mitochondrial complex I and ACO2 were detected in the human LIHC cell lines and hepatocytes, and the histogram represented the relative activity of control (hepatocytes). Data are presented as means ± SD (n = 3). *, p < 0.05; **, p < 0.01