Fig. 5

Lnc-Ma301 inhibits the lung metastasis ability of HCC cells in vivo. A A lung metastasis model was established by injection of SMMC-7721 HCC cells into the tail vein of nude mice, and lung metastasis was investigated. Overexpression of lnc-Ma301 was confirmed by qRT-PCR before injection. Photographs of lung metastasis were taken (blue arrow indicates tumor nodule). Amagnified lung in each group is shownas an example. B Histological examination of lung colonization. Magnification, × 200. Expression of E-cadherin, MMP9, and Ki67 in the lung tissues was quantified by qRT-PCR. C Immunohistochemistry was used to validate the protein levels of E-cadherin, MMP9, and Ki67 in each group. Western blotting was also used to confirm the protein levels of E-cadherin, MMP9, and Ki67. Experiments were performed in triplicate in each experiment. *P < 0.05