Fig. 2

Effect of EEPK on disrupted mitochondrial membrane potential, cytochrome c release, and antiapoptotic Bcl-2 family proteins. MC3 and YD-15 cells were treated with DMSO or the indicated doses of EEPK for 24 h. a Measurement of mitochondrial membrane potential in human MEC cells with or without EEPK using a JC-1 probe. Bar graphs represent the mean ± SD of three independent experiments. ^*p < 0.05. b Cytochrome c release into the cytosol was evaluated using cytosolic and mitochondrial fractions. Cox IV and α-tubulin were used as specific fraction markers for mitochondria and cytosol, respectively. Data are representative of two independent experiments. c Western blot images showing the expression levels of Mcl-1 and Bcl-2. β-actin was used as an internal control. All bar graphs represent the mean ± SD of three independent experiments. ^*p < 0.05