Fig. 3

Effect of EEPK on Mcl-1 protein turnover in human MEC cells. a MC3 and YD-15 cells were treated with DMSO or the indicated doses of EEPK for 24 h. Relative mRNA levels of Mcl-1 in EEPK-induced human MEC cells were assessed using qPCR and normalized to GAPDH. Data are shown as the mean ± SD of three independent experiments. b The protein levels of p-eIF4E were examined with western blotting. β-actin was used as an internal control. All bar graphs represent the mean ± SD of three independent experiments. ^*p < 0.05. c Both cells were pretreated with the indicated doses of CHX for 1 h in the absence or presence of EEPK for 24 h. Representative western blot images for Mcl-1 expression. d Pretreatment with the indicated doses of MG132 for 1 h in the absence or presence of EEPK for 24 h. The protein levels of Mcl-1 were determined via western blotting. β-actin was used as an internal control. All bar graphs represent the mean ± SD of three independent experiments. ^*p < 0.05; ^#p < 0.05