Fig. 4
Effect of EEPK on phosphorylated Bcl-2 (Ser70) through the activation of the JNK signaling pathway. MC3 and YD-15 cells were treated with DMSO or indicated doses of EEPK for 24 h. a Western blot images showing p-Bcl-2 (Ser70) expression levels. b The p-JNK and JNK expression levels were analyzed with western blotting. β-actin was used as an internal control. c Both cells were pretreated with the indicated doses of SP600125 for 1 h and then treated with DMSO or EEPK for 24 h. Representative western blot images showing the expression levels of p-JNK, JNK, and p-Bcl-2 (Ser70). β-actin was used as an internal control. All bar graphs represent the mean ± SD of three independent experiments. *p < 0.05; #p < 0.05